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Does anyone have experience/knowledge of expressing bacterial (or other microbial) proteins in mammalian cells? I am interested in this for a project where we are trying to introduce a new enzymatic activity in human cells. I have been looking for examples in the literature, but haven't found anything so far.

I can imagine that various problems might arise. Presumably transcription and translation should not be a problem; but I can imagine the protein may not get sorted/localized properly, or might not be properly degraded and so could accumulate to toxic levels ... or what not.

Any advice or pointers to literature would be much appreciated!

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