For my science fair project, I did an experiment on how paracetamol and ibuprofen affected the growth of bacteria. By day 5, however, parts of the paracetamol blackened (image below). I am wondering if E. Coli can metabolize paracetamol and if so, what operon in the K-12 genome is responsible for this.
EDIT: I realized it may be metabolizing other ingredients in the medicine I used. I am including a list of the ingredients in the medicine I used.
Acetaminophen 160 mg in each 5 mL (duh)
Other ingredients (33.015873 mL):
- Anhydrous citric acid
- FD&C red no. 40
- high fructose corn syrup
- microcrystalline cellulose and carboxymethylcellulose sodium
- purified water
- sodium benzoate
- sorbitol solution
- xanthan gum
I thought it might be the fructose, but is the byproduct really black? Still confused...