I have a large ( >100 ) list of accession numbers I want to look up and match to searches in NCBI (nucleotide); mainly for getting a tentative organism to match to the accession number.
KJ841938.1 would match to Setoptus koraiensis ... FJ911852.1 would match to Uncultured eukaryote ...
I googled for tools, and I found this site. However, it is not what I really want since it doesn't list my queries in the same order as my list, which means I cannot match.
I also attempted to write a script in biopython using Entrez E-tools, but was unsuccessful due to a lack of coding skill.
Does anyone have any way I can go about this?
EDIT: Via this tutorial, I attempted to use this code sample:
from Bio import Entrez Entrez.email = "Your.Name.Here@example.org" handle = Entrez.efetch(db="nucleotide", id="AY851612", rettype="gb", retmode="text") print(handle.readline().strip()) # expected output : LOCUS AY851612 892 bp DNA linear PLN 10-APR-2007 handle.close()
But modified to take any list instead of the
variable id, as below:
import Bio print (Bio.__version__) from Bio import Entrez import time Entrez.email = "Your.Name.Here@example.org" id_list = ["KJ841938.1", "FJ911852.1"] # real list is about 500 elements x = 0 while x < len(id_list): handle = Entrez.efetch(db="nucleotide", id=id_list[x], rettype= "uilist", retmode="text") #print(handle.readline().strip()) print(handle.readline()) handle.close() x = x + 1
1.69 673539906 283462561
However, I do not believe I am using the right "rettype" parameter in the
.efetch function as I keep on getting GI numbers, where as I was hoping to get something like a species name directly. Unless I can then search with these GI numbers in batch with more code or a tool to produce a ordered list?