Where is DNA during the process of RNA extraction using Trisure described here? Given that DNA is also a nucleic acid, should I expect the isolated RNA to also contain DNA? However, Bioline claims RNA 'with virtually no genomic DNA contamination'.
Expectedly, they don’t really want to tell you what TRIsure is. What they do say is that DNA is found in the organic or interphase after extraction whereas RNA partitions to the aqueous phase. This is almost certainly some variant of acid phenol extraction. As confirmation, the MSDS lists phenol, ammonium thiocyanate, guanidinium thiocyanate, glycerol and citric acid as its components.