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I am working with C. elegans and bacteria and I want to get rid of the bacteria they eat by centrifuging the worms without centrifuging the bacteria. I am using a g-force of 600 and the bacteria sometimes still centrifuge down. What g-force would be ideal to pellet the worms but not the bacteria? Does this depend on the bacterial species?

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  • $\begingroup$ Have you tried using Ficoll to separate them? $\endgroup$ – CKM Feb 9 '18 at 14:04
  • $\begingroup$ no, how does it work? The wikipedia page mentions its use for blood cells separation. $\endgroup$ – charlesdarwin Feb 9 '18 at 14:11

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