When making competent cells I've been using a medium called "Psi broth", which per liter consists of 20 g tryptone, 5 g yeast extract and 5 g MgSO4. Today I got curious about why it's called "psi". Is it an acronym like SOC and LB?
Psi is the Greek letter ψ. Psi broth is sometimes referred to as ψ-broth.
Here's an example:
L-Broth or ψ-broth can be used instead of SOC medium, but efficiency may be reduced.
- L-broth： 10 g Bacto tryptone, 5 g Bacto yeast extract, 5 g NaCl, pH to 7.5 with 1 M NaOH, bring to a final volume of 1 L, and autoclave.
- ψ b-broth： 5 g Bacto yeast extract, 20 g Bacto tryptone, 5 g MaSO4･7H2O, pH to 7.5 with 1M KOH, bring to a final volume of 1 L and autoclave.