I am trying to self study BNDF a little bit when I found this article:

Abstract Stress impacts multiple systems within the CNS, including the hippocampus. A key molecular mediator in hippocampal functions, including learning and memory, is brain-derived neurotrophic factor (BDNF). BDNF mRNA is reported to be decreased following chronic (every day) immobilization stress (CIS) in the rat, however the effect on protein is unknown. Utilizing a mouse model of CIS, the hypothesis that total hippocampal BDNF protein, in addition to BDNF mRNA, would be decreased was tested. The effects of intermittent (alternating day) immobilization and cold stress on hippocampal BDNF protein levels was also examined.

In this study it was demonstrated that CIS results in a small (8 per cent decrease relative to non-stressed controls), but significant (p = 0.0013) decrease in the exon coding for mature BDNF (exon VI). In addition, there was a significant (34 per cent, p = 0.005) decrease in the mRNA coding for exon I in the stressed animals. Contrary to expectations, however, CIS resulted in a significant (116 per cent, p = 0.0087) increase in hippocampal BDNF protein levels. Furthermore, both intermittent immobilization and chronic cold stress both increased BDNF protein levels. Chronic stress, therefore, differentially regulates BDNF mRNA and protein levels in the hippocampus. Copyright © 2004 John Wiley & Sons, Ltd.

I'm really curious what they mean with CIS: Chronic immobilization stress. I can't find anything on the internet that actually explains what this is.


1 Answer 1


There are >1200 articles tagged "chronic immobilization stress" on pubmed.

From Hennebelle et al. (2012):

Half of the 6-month old male rats from each dietary group were subjected to a validated chronic stress procedure (Buyntsky and Mostosky, 2009) based on a restraint stress procedure adapted from Veena et al, [32]. Rats were placed in wire mesh restrainers for 6 h a day (fom 10 am to 16 pm) for 21 days (5 days a week) over a period of 4 weeks. Unstressed rats were handled weekly. The unstressed and stressed animals were housed in different rooms.

Hennebelle, H., et al. 2012. Influence of Omega-3 Fatty Acid Status on the Way Rats Adapt to Chronic Restraint Stress. PLoSOne 7: e42142.

  • $\begingroup$ So Chronic Immmobilization Stress means they don't allow (the rats in most cases) to move freely ? $\endgroup$
    – Kasper
    Commented Feb 6, 2013 at 14:13
  • $\begingroup$ Yes for certain period of time. $\endgroup$
    – kmm
    Commented Feb 6, 2013 at 16:49

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