I have been told that interphase cells are better for gene mapping purposes than metaphase cells but don't know why.

  • $\begingroup$ Can you give more information about what kind of mapping is being done? Do you have a reference for the claim? $\endgroup$ – Remi.b Mar 10 '18 at 15:42
  • $\begingroup$ Clones of human DNA were used for FISH. I don't have a reference for the claim, but please could you suggest some advantages of using interphase cells rather than metaphase cells for gene mapping. $\endgroup$ – Bio314 Mar 10 '18 at 20:11
  • $\begingroup$ Already good to know the question is about FISH. You should edit this info in your question. Have you read the wikipedia on FISH? There seems to have quite a bit of info already that may well help you. $\endgroup$ – Remi.b Mar 10 '18 at 20:31
  • $\begingroup$ Thanks for your reply. The wiki page describes the process of FISH but doesn't address advantages of using interphase instead of metaphase cells. $\endgroup$ – Bio314 Mar 10 '18 at 20:35

In metaphase, chromosomes are at their highest level of condensation. FISH relies on being able to dissociate the two strands of DNA to anneal a sequence-specific probe. So, my guess is that it is technically easier to achieve a good FISH staining in interphase, when chromosomes are much less densely condensed. There might be other reasons I am not aware of (I am not a geneticist).


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