In an experiment, I recently isolated and purified calf thymus DNA, hydrolyzed it with perchloric acid, and then applied the hydrolyzate to a Whatman paper in a polar solvent and let it run overnight.

I must now determine a way to quantify the concentration of each base, now separated based on polarity on the chromatogram. This cannot be the following method: Cutting the paper containing the base and putting it into an acid-containing flask, then measuring the absorbance, followed by Beer-Lambert's Law calculation for the concentration.

I would appreciate any ideas.



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