1
$\begingroup$

In an experiment, I recently isolated and purified calf thymus DNA, hydrolyzed it with perchloric acid, and then applied the hydrolyzate to a Whatman paper in a polar solvent and let it run overnight.

I must now determine a way to quantify the concentration of each base, now separated based on polarity on the chromatogram. This cannot be the following method: Cutting the paper containing the base and putting it into an acid-containing flask, then measuring the absorbance, followed by Beer-Lambert's Law calculation for the concentration.

I would appreciate any ideas.

$\endgroup$

closed as off-topic by David, Bryan Krause, AliceD Mar 23 '18 at 22:03

This question appears to be off-topic. The users who voted to close gave this specific reason:

  • "Homework questions are off-topic on Biology unless you have shown your attempt at an answer. For more information see our homework policy." – David, Bryan Krause, AliceD
If this question can be reworded to fit the rules in the help center, please edit the question.