I haven't seen any gel lanes in neither my PCR samples nor the marker itself when photographing them. This is not my first time performing 1.2% agarose gel electrophoresis.
I see gel lanes when I take the gel out the apparatus, but when I go to photograph them: nothing shows up. I only let the samples "run" for 30-40 minutes (when I ran for an hour, the bands almost ran off the gel).
Tried using different markers: no luck.
Re-added some more EtBr to better stain the gel: no luck.
I am also keeping the lights off in the dark room, so I do not think I am exposing the film to light early on (errors still a possibility though).
What could I be doing wrong? Am I taking the film out too early?