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I've been thinking of culturing Cutibacterium acnes (old name is Propionibacterium acnes) for a thesis topic of mine on the comparison of the efficacy of local antibacterial topical skin care products but I have no idea how to culture it. I haven't encountered any definite instructions on the process in culturing it aside from it being an aerotolerant anaerobe and requring an anaerobic incubator.

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Douglas & Gunter (1946) The Taxonomic Position of Corynebacterium acnes. J Bacteriol 52: 15-23.

This is the paper, in which Corynebacterium acnes was proposed to be transferred to the genus Propionibacterium as Propionibacterium acnes, and it contains a detailed description of the cultivation procedure.

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