In my bio class, we learned that you can use restriction enzyme or PCR to linearize a plasmid. Linearizing by restriction enzyme seemed visual and intuitive but how do one linearize a vector using PCR? I know how PCR works(at least I believe I do), since I watched couple of videos on YouTube explaining them.
But it is not connecting to me how PCR can be used to linearize a "circular" DNA. I have seen videos where a straight double stranded DNA was separated into single strands and using PCR to produce multiple copies with appropriate primers.
But if a plasmid, the circular DNA is not "straight" or linearized in the first place how do you perform PCR on it? Because if they are in a circular state during PCR the strand would never separate in to linear strands. Wouldn't you just have two sperated strands that are circular? E.g a small ring of single strand DNA and a bigger ring of single strand DNA. They are still in circular state after the strands split from each other. Please correct me if I am spitting out nonsense. I just don't see the visual here.