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We're expressing a protein in a model organism for crystallization, however it seems the protein has some toxic effect on the cells.

Is there something we can do to make it non-functional during expression so it won't affect production, in such a way that we can restore function later (ex. after purification) in a simple way?

I know there are other approaches available but I'd like some inputs on this one alone.

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  • $\begingroup$ That would depend on the specific protein being expressed. You could try secreting the protein or targeting it to inclusions and then refolding. $\endgroup$ – canadianer Apr 17 '18 at 15:48
  • $\begingroup$ Can you provide more information? Are you expressing the protein in yeast or bacteria? $\endgroup$ – user22542 Apr 17 '18 at 16:00
  • $\begingroup$ @user22542 in yeast, however I'd be interested to hear approaches that can work in different vectors too $\endgroup$ – SilverCookies Apr 18 '18 at 16:38
  • $\begingroup$ I assume the toxicity is limited and not lethal. Is the protein showing the toxic effect being secreted during production or retained in the cells? $\endgroup$ – user22542 Apr 18 '18 at 21:05
  • $\begingroup$ @user22542 It is secreted in the medium $\endgroup$ – SilverCookies Apr 19 '18 at 7:56

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