I am currently confused about the estimation of multiplicity of infection (MOI), here is an example in a scientific article
in figure 3b, they tested the virus, and get estimated MOI based on this:
To estimate the MOI, we isolated a total of 45 individual neurons from three animals injected with MAPseq virus into the right LC and sequenced the barcodes within each neuron. On average, infected LC neurons contained 1.2 ± 0.1 barcodes each, implying MOI of 0.43 (Figure 3B).
I am confused which equation they used for estimating?
Thanks in advance!