Is there any way to increase the contrast of my SDS PAGE gel. I want to increase the coomassie stained gel contrast of my gel bands a little bit as it looks little less for my thesis. Ive heard that it is legal and can be done. Is there any software for it or some other way.
If this is accepted or not depends on your institution. For publications, it is usually accepted to use adjustions of color, tonal values and contrast which are applied to the whole image and which do not alter the statement of the image. These changes are not ok if for example the contrast it raised so much that weak bands disappear.
Not accepted are changes which are only made to changes to parts of the image, partial adding or removal of parts of the image. Keeping this in mind, you can basically use any software which can be used to process images. Photoshop is kind of a standard but pretty expensive, free software like Gimp also does the job.
I adjusted the tonal values of your image and raised the contrast just a little to get this: