I am a graduate student looking to incorporate some protein engineering into my training. I have been reading a lot about E coli lysate based systems, but they still require 3rd party additives like amino acids etc.. ($$$). I need this to be ultra cheap as this will be a side show until I can show something for my efforts. I prefer a cell free workflow because of the quick turnaround from template to protein and ease of purification. My backup plan is just to add a tag at the end of my constructs but I would prefer to avoid this.
Any suggestions would be much appreciated.