I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of alpha-amylase), 500µg/ml(37%),750µg/ml(46%) and 1000µg/ml(63%). Basically, alpha-amylase results in increase in glucose level after eating food. So i wanted to know that how could i find out that what is the best concentration of my plant extract to be used as drug.
Usually there are multiple clinical trials, first in animals, then in humen to
- figure out the effective dose
- look for side effects
- compare the new drug's efficacy with existing drugs