I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of alpha-amylase), 500µg/ml(37%),750µg/ml(46%) and 1000µg/ml(63%). Basically, alpha-amylase results in increase in glucose level after eating food. So i wanted to know that how could i find out that what is the best concentration of my plant extract to be used as drug.
You have to try to determine the best concentration.
Usually there are multiple clinical trials, first in animals, then in humans to
- figure out the effective dose
- look for side effects
- compare the new drug's efficacy with existing drugs