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I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of alpha-amylase), 500µg/ml(37%),750µg/ml(46%) and 1000µg/ml(63%). Basically, alpha-amylase results in increase in glucose level after eating food. So i wanted to know that how could i find out that what is the best concentration of my plant extract to be used as drug.

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  • $\begingroup$ This sounds like a homework question. Is it? $\endgroup$
    – David
    Commented Jun 9, 2018 at 8:05
  • $\begingroup$ No, i am in my final year of my btech working on a plant and when a plant report some property then it is better if we can also give its best concentration. $\endgroup$
    – roxaite
    Commented Jun 9, 2018 at 8:11

1 Answer 1

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You have to try to determine the best concentration.

Usually there are multiple clinical trials, first in animals, then in humans to

  1. figure out the effective dose
  2. look for side effects
  3. compare the new drug's efficacy with existing drugs
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  • $\begingroup$ I think this is better as a comment as it doesn't answer the question which in any case looks very like a homework question. $\endgroup$
    – David
    Commented Jun 9, 2018 at 8:08
  • $\begingroup$ @David Actually I started writing a comment first and was expecting a "please don't answer in comments" response :) And it didn't occur to me this could be homework. $\endgroup$
    – Arsak
    Commented Jun 9, 2018 at 8:11
  • $\begingroup$ @David Owh, and the answer was: You have to try. $\endgroup$
    – Arsak
    Commented Jun 9, 2018 at 8:14
  • $\begingroup$ "humen"? spelling is human... $\endgroup$
    – Solar Mike
    Commented Aug 9, 2019 at 8:06
  • $\begingroup$ @SolarMike Yeah, was confused by the man/men and woman/women plural. $\endgroup$
    – Arsak
    Commented Aug 9, 2019 at 8:22

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