For 24 well plate wells, thermofischer recommends to seed 0.05E6 cells with 0.5 to 1 ml of medium. What are recommended numbers for a 384 well plate well?
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The Privalsky Lab@ UCDavis writes:
Growth Area of Tissue Culture Plates and Dishes
Growth Area (cm2) Media Volume (ml) Maximum Volume (ml)
Plates
35 mm 8 1.6- 2.4
60 mm 21 4.2- 6.3
100 mm 44 11 -16.5
150 mm 148 30 -45
245 mm (square) 500 100-150
Dishes
6 well 9.5 1.9 -2.0 16.8
12 well 3.8 0.76 -1.14 6.9
24 well 1.9 0.38 -0.57 3.4
48 well 0.95 0.19 -0.28 1.6
96 well 0.32 0.1 -0.2 0.36
384 well 0.056 0.025-0.05 0.125
Flasks
T25 25 5- 7.5
T75 75 15-22.5
T150 150 30-45
