0
$\begingroup$

How can I blast my BSP(Bisulfite sequencing) primers? I designed in this way that C nucleotide converted to T and my primers just sit on the converted DNA! I blast it with Bisearch but I don't know how can I blast in with NCBI!!!! thanks.

$\endgroup$
  • $\begingroup$ Hello! What is your goal? Do you want to know what is the possible amplified target from your primers? How did you designed your primers? $\endgroup$ – LinuxBlanket Jun 21 '18 at 12:48

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.