How can I blast my BSP(Bisulfite sequencing) primers? I designed in this way that C nucleotide converted to T and my primers just sit on the converted DNA! I blast it with Bisearch but I don't know how can I blast in with NCBI!!!! thanks.

  • $\begingroup$ Hello! What is your goal? Do you want to know what is the possible amplified target from your primers? How did you designed your primers? $\endgroup$ – LinuxBlanket Jun 21 '18 at 12:48

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