I am trying to work out whether the enzyme Tax10 is soluble or insoluble. I need to know if some buffers won't work with Tax10. I am trying to confirm Tax10 activity, having confirmed protein expression and having purified it. Any help would be much appreciated! Thanks :)
The TAX10 gene overexpressed from pSBET yielded a ≈50-kDa protein (determined by SDS/PAGE), consistent with the size of the encoded enzyme, and this operationally soluble recombinant enzyme from preparative bacterial cultures was partially purified by anion exchange (DE-52) and ceramic hydroxyapatite chromatography.
(p.9169, below Figure 3)