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I am trying to work out whether the enzyme Tax10 is soluble or insoluble. I need to know if some buffers won't work with Tax10. I am trying to confirm Tax10 activity, having confirmed protein expression and having purified it. Any help would be much appreciated! Thanks :)


Context:

Walker K, Long R, Croteau R. 2002. The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus. Proc Natl Acad Sci USA 99(14):9166-9171.

The TAX10 gene overexpressed from pSBET yielded a ≈50-kDa protein (determined by SDS/PAGE), consistent with the size of the encoded enzyme, and this operationally soluble recombinant enzyme from preparative bacterial cultures was partially purified by anion exchange (DE-52) and ceramic hydroxyapatite chromatography.

(p.9169, below Figure 3)

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  • $\begingroup$ I think all they mean is that the protein was found in the soluble fraction rather than in inclusions. $\endgroup$ – canadianer Jun 29 '18 at 19:26
  • $\begingroup$ Fantastic. I thought that's what they meant. Thanks :) $\endgroup$ – derek1984 Jul 2 '18 at 19:00

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