so I have to 'quantitatively' assess a bacterial strain for its antibiotic resistance property (it is a specific antibiotic) in the presence and absence of another compound alongside antibiotic. What technique or protocol can be followed?


1 Answer 1


It sounds like you might employ a "Disk diffusion test" described in this Wikipedia article. It could be done with and without the secondary adjuvant compound and be quantified by measurement of the varying diameters of the resulting zones of bacterial inhibition.


  • $\begingroup$ thank you that was very helpful but the page does not describe how to add adjuvants in the equation. I googled for it and still couldn't find a satisfactory answer $\endgroup$
    – user154547
    Commented Jul 9, 2018 at 12:45
  • $\begingroup$ I suppose you would add them to the mueller-hinton when making the plate, a few for each concentration of the other compound you want to test. Then run the disk diffusion as usual. $\endgroup$
    – Eliane B.
    Commented Jul 9, 2018 at 13:11
  • $\begingroup$ Eliane B - obviously. The question involves the addition of a potential adjuvant (other compound). It's addition can be determined by the experiment. $\endgroup$
    – user22542
    Commented Jul 9, 2018 at 13:30

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