Upon reading a report today, I discovered a interesting technic of plasmid transformation. Triparental Conjugation, a bacterial conjugation including three parts , a conjugative plasmid, a donor cell ,and a giver cell. The question is that under what circumstances should we implement this technic? What is difference between the normal heat shock and triparental conjugation?
(The Wiki information page as well doesn’t depicted the reason clearly https://en.m.wikipedia.org/wiki/Triparental_mating )
Thanks in advance!