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Is it possible to estimate population divergence time with a single mitochondrial marker?

Here is additional information for my specific case:

  • Mitochondrial marker: Cytochrome Oxidase Subunit 1 (COI)
  • Molecular clock rate for COI for my taxon of interest: 1.29-1.96 % per million year
  • Age of the taxon: ca. 2.69 million years
  • Number of individuals per population: 10
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