We are trying to find biomarkers on the B-Cell receptor VDJ regions. To do this we plan to run Single cell RNA-Seq (using the solution from 10x genomics https://www.10xgenomics.com/solutions/vdj/) to get the transcripts, then assembling these using VDJ Puzzle: https://www.biorxiv.org/content/early/2017/08/26/181156
The output will be a list of about 100k base pairs per patient per cell sequenced. I will then have clinical information ... how can I compute the number of patients needed to establish a statistically significant association between SNPs and clinical outcomes / disease state?
Furthermore how many cells would we need to sequence per patient?