If I am treating an organism in MnCl2 dissolved in water, how do I determine if the amount of Cl (in MnCl2) will change the neural resting potential Oor if it will influence motoneurons?


2 Answers 2


For theoretical calculations with other ions, you need to know the concentration and permeability of each of the major ions inside and outside the cell, both with and without the added substance (in your case MnCl2), and then use the Goldman equation/GHK equation to calculate resting potential before and after. Approximations would probably be sufficient.

However, chloride can be a bit complicated because the intracellular chloride concentration can fluctuate with extracellular chloride moreso than the other major ions. Therefore, depending on the sensitivity of your experiment, you may need to perform whole-cell patch clamp recordings to measure the resting potential directly.

If the change in chloride is a small percentage of the total, you can probably consider the influence to be negligible.


As the previous answer states you will need to know, or estimate, the concentration of ions and their relative permeability.

Knowing these values you can estimate the change using the following link:


Do you only have MnCl2 in your solution? Are there any other ions?

  • $\begingroup$ My solution consists of MnCl2 in water $\endgroup$
    – user45842
    Oct 2, 2018 at 16:23
  • $\begingroup$ Then you will greatly affect the resting potential. Probably since you don't have potassium or sodium outside you'll generate an hyperpolarization. $\endgroup$
    – BPinto
    Oct 2, 2018 at 17:50

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