Different domains of DNA polymerase contain different activity, like
5'->3' polymerisation and
3'->5' proof reading activity (a general case), and these domains can be exploited separately to make them perform single activity at a time. For example if we are conducting detection of some sort of mutation on a strand of DNA, we need to suppress the activity of DNA polymerase proof reading, ie, stopping it to correct the error it makes.
Since the proof reading activity increases the fidelity of replication to some 99.9%, I want to know what would be the efficiency if there is no proof reading activity.
To simplify my question- If DNA repair system commits 1 mistakes out of 10^8 base pairs it added in nucleotide chain, how many errors would occur if we remove it's proof reading mechanism?