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I'm looking for books and articles that can bring me up to speed on the design, expression, and purification of mammalian proteins in recombinant systems, both in E. coli and in more complex systems like CHO or Sf9 cells. I have a weak but functional background in general molecular biology.

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  • $\begingroup$ Hello and welcome to Bio.SE! Take the tour to learn about the site and earn yourself your first badge. Have a nice stay! $\endgroup$ Oct 13, 2018 at 12:26

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i would start by searching PubMed literature database for relevant articles using this query:

"protein"[Title/Abstract] and "expression"[Title/Abstract] and "purification"[Title/Abstract] and "recombinant"[Title/Abstract] and "mammalian"[Title/Abstract] and ("E. coli"[Title/Abstract] or "CHO"[Title/Abstract] or "Sf9"[Title/Abstract])

which returns 116 articles (including 7 reviews).

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  • $\begingroup$ Hello! This question was deemed worth of an answer, so why not upvote it? Vote early, vote often to encourage new users and keep the community alive! $\endgroup$ Oct 13, 2018 at 12:26
  • $\begingroup$ @LinuxBlanket Except that it is not an answer but a suggestion as to how to find an answer. $\endgroup$
    – David
    Dec 7, 2019 at 13:10
  • $\begingroup$ @David "why not upvote it" - "it" was referred to the question, not the answer. I wanted to incite the answerer to upvote the question. I didn't express myself clearly, my bad! $\endgroup$ Jan 31, 2020 at 16:16

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