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I have been working on lung samples to perform western blotting. I have two related questions about this:

  1. I get very nonspecific results: at times nothing at all, at times it glows on membrane but doesn't take up on x-ray film. What are the common causes of these errors and how might I avoid them?

  2. I am interested in assaying GPCR expression. When I am assaying for GPCRs, do I need to prepare the lung samples in a specific way (that is different from sample preparation for Western blots of other proteins)?

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  • $\begingroup$ You may be interested in this: biology.stackexchange.com/questions/7205/… $\endgroup$ – blep Apr 8 '13 at 3:38
  • $\begingroup$ I wrote the answer to the above question, please let me know if you're still having issues. Just leave a comment with @MattDMo in it. $\endgroup$ – MattDMo Apr 8 '13 at 15:36