I have been working on lung samples to perform western blotting. I have two related questions about this:
I get very nonspecific results: at times nothing at all, at times it glows on membrane but doesn't take up on x-ray film. What are the common causes of these errors and how might I avoid them?
I am interested in assaying GPCR expression. When I am assaying for GPCRs, do I need to prepare the lung samples in a specific way (that is different from sample preparation for Western blots of other proteins)?