Phi29 polymerase is often used at 25-30 degrees Celsius and heat inactivated at 60-70 degrees. I couldn't find any data on its functionality on temperatures around 40-50 degrees. Before testing it myself I want to ask this community wheter someone has used it successfully at such high temperatures.


Your Answer

By clicking "Post Your Answer", you acknowledge that you have read our updated terms of service, privacy policy and cookie policy, and that your continued use of the website is subject to these policies.

Browse other questions tagged or ask your own question.