In dealing with the question above I know that if we plot 1/v against 1/[isocitrate] we can get V-max = 1/intercept and Km = slope/intercept.

However when I express the two rows of values in micromoles I get intercept = 3359, and I believe it will be in micromoles? That gives me V-max = 1/(3359*10^-6) = 297.7. However, how do I know the order of magnitude of this number? The textbook says it is pmol, but how is it picomoles/ dm3 s? enter image description here


closed as off-topic by David, kmm, Chris Nov 22 '18 at 8:55

  • This question does not appear to be about biology within the scope defined in the help center.
If this question can be reworded to fit the rules in the help center, please edit the question.

  • 2
    $\begingroup$ I'm voting to close this question as off-topic because this is not about Biology but numerical manipulation, and in a specific instance. There are many resources on the web for this sort of thing. $\endgroup$ – David Nov 18 '18 at 16:01
  • $\begingroup$ Another more general point. Do not post text as images. It discriminates against people with sight disabilities. $\endgroup$ – David Nov 18 '18 at 16:03

The order of magnitude for your intercept value depends on the axis of your graph, which in turn depends on the values / units of your input values.

As an example if your substrate concentrations are in the range of $\mu mol / l$ (which is micromolar, not mircomoles), then that is also the order of magnitue you'll get out of the calculation.

$dm^{-3}$ equals litre's ($1dm^{-3} = 1l$), it's not usually not used for concentartions, so I can't say why the textbook used it instead. Maybe they wanted to use base SI units.


Not the answer you're looking for? Browse other questions tagged or ask your own question.