I have a problem with my proteomics homework. I was asked to design primers used to create a homologous recombination box to create PRP24-TAPtag yeast. I know that homologous regions should be 40-50nt and that my primer should include overhangs. I was told to base off of this publication: https://www.ncbi.nlm.nih.gov/pubmed/11403571

I have designed primers before, but this time I have encountered really big problems. I have found both my plasmid, pBS1479, TAPtag and PRP24 protein sequences, but I have no idea how should I join them to create primers and how to include the TAPtag. This is my first time using this method. Are there any tools I can use for this task? Should I add tag sequence to C end of my protein, then use plasmid seqence to create overhangs?


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.