What are the limitations of using Illumina's smaller sequencers (e.g., iSeq100, miniSeq) to do de novo whole genome sequencing on an organism with a larger genome (~2Gb)?

Naively, it seems even if I am only able to do 5Mb worth of sequencing per 24 hour run on these sequencers, after about two years of running the thing continuously, I should be able to have enough reads to be able to assemble a de novo genome sequence of a larger eukaryote (downstream bioinformatics concerns aside). Yet clearly the sequencers are never marketed like this and I'm struggling to understand why.

I am interested in GWAS on non-model vertebrates if that matters. That time frame doesn't seem crazy, as some field experiments take several years before something is publishable.

Yet clearly the sequencers are never marketed like this and I'm struggling to understand why.

You don't see why two years of instrument reagent costs for a single project might be considered prohibitive?

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