I am a student, so I hope it is okay to put this question here. I've done the virus plaque assay in a practical recently and have a few questions regarding the plaque count for pfu calculation.
In the plates corresponding to times 0 and 10 mins, a few plaques formed (less than 5 plaques in every plate where plaques could be seen) Should I still calculate pfu with those results, or should I leave it as 0 pfu, and use only plaque counts that are at least more than 10?