Can I use just a primer and PCR to join the cutted plasmid like this ? without using any further enzyme like ligase.
After the linear dsDNA plasmid annealed into 2 ssDNA, primer will bind to middle. And Taq Polymerase will bind to the 3' and start extend the DNA to join the plasmid. So I don't need a ligase enzyme, is that possible?
Edit: I think the plasmid will be nicked at 2 position with this method but will this plasmid still work in e.coli cell?