5
$\begingroup$

There are many genetically engineered foods and animals and I was wondering, how do scientists know which part of a DNA to cut in order to produce the desired result. For example I want to make a tomato more durable and to do that I cut the durability part of the DNA of something long lasting and put in the tomato. I'm not sure, but I guess that's not how things work. If I wanted to make a human with tiger arms, I'll just cut the arms DNA part of the tiger and put it in the human DNA, no? I'm sure it's much much more complicated than that.

P.S - I'm just 16 and interested in biology, I do not posses any university/professional academic knowledge.

$\endgroup$
5
$\begingroup$

This is a tough question (scientifically)! Generally speaking, it's impossible to look at a DNA sequence and predict what function it might have. It's easier to go backwards: identify a function/trait of interest, figure out what proteins are necessary for that function, and then deduce the sequence.

I'll describe one strategy to go from protein to gene, and name some techniques which you can read up on if you want to learn more.

To find the amino acid sequence of a protein, you can chop it up using proteolytic enzymes. This gives you fragments of varying lengths, since the enzymes will cut semi-randomly at different sites in the protein. Run these fragments on chromatographic paper. You can deduce the amino acid sequence from the pattern, because larger fragments will move slower. This is the same idea behind Sanger sequencing (aka chain termination sequencing) of DNA, and was invented by the same guy.

If you know what the amino acid sequence is, you now know the mRNA sequence, since protein is translated from mRNA. However, in eukaryotes, you don't know the DNA sequence, since we have introns - i.e. bits of the sequence which get cut out between transcription and translation. We need a way to get from mRNA (protein precursor) to DNA (gene).

To do this, purify mRNA from a cell and generate a library of complementary DNA (cDNA) using reverse transcriptase. Then, sequence the cDNA library to generate expressed sequence tags (ESTs), which are subsets of the full cDNA sequence. Because the ESTs are subsets of the sequence instead of the full transcript, it's possible to "CTRL+F" the organism's genome and find the gene which contains the EST.

Scientists have been doing this for decades, so nowadays you can search a gene/protein database and instantly know which sequence is responsible for, say, a specific channel protein.

To go in the other direction from gene to protein to function/trait, you have to do a fair bit of legwork as well. In very, very simplistic terms, you "cut" some DNA, put it into a package, and deliver the package to your favorite organism. As you said, it's a bit more complicated than that. :)

For complex, polygenic traits, it's harder to (1) identify the genes responsible and (2) successfully engineer an organism to have all of those new genes. You're unlikely to see a human with tiger legs any time soon. It's much easier to give an organism a few genes that confer very specific traits. Look at this list of genetic modifications to crops and click on one of the traits; usually only 1-3 genes have been introduced.

$\endgroup$
  • $\begingroup$ This answer feels incomplete and pretty much skips over the very important work in genetics done in lower organims like yeast to identify the function of genes. $\endgroup$ – Cell Dec 26 '18 at 14:09
  • 2
    $\begingroup$ @Cell thank you for pointing this out. I felt this particular user was looking for a simple explanation that they could visualize, so I chose to describe one specific approach. In fact, I started writing this answer in response to a different question that the user asked shortly after this one, but the other question was so broad that my answer was better broken into pieces. I'll edit my response today to give a brief sketch of yeast genetics and am welcome to further suggestions. $\endgroup$ – aeffenberger Dec 26 '18 at 15:35
3
$\begingroup$

I will name one classical methodology to associate genetic variation in population to phenotypic variation.

We can figure out functions of specific sequence thorugh correlation analyses. We sample a number of individuals from the population, sequence them (or genotype them) and measure a phenotype of interest. Then, we screen through their genome and for each locus (locus = position in the genome) we investigate whether there is a correlation between the genetic variation at this locus and the phenotypic variation. It is classic to represent these correlation tests throughout the genome with a Manhattan plot

enter image description here

, where the Y axis is the negative of the log of the P.value and the X axis is the position in the genome. From the above plot, one can tell that several loci at the end of the 19th chromosome are associated with the phenotype of interest. There is therefore likely at least one SNP over there that affects this phenotype.

This type of analysis are called Genome-Wide Association analyseS (GWAS; pronounced G-wass).

$\endgroup$

Your Answer

By clicking "Post Your Answer", you acknowledge that you have read our updated terms of service, privacy policy and cookie policy, and that your continued use of the website is subject to these policies.

Not the answer you're looking for? Browse other questions tagged or ask your own question.