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Isn’t it easier to use a 1000 µl micropipette tip for all volumes? I don’t understand why there are different tips for smaller volumes. Does it affect the accuracy of the pipette for very small (<5µl) samples?

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closed as off-topic by David, WYSIWYG Jan 10 at 11:03

  • This question does not appear to be about biology within the scope defined in the help center.
If this question can be reworded to fit the rules in the help center, please edit the question.

  • $\begingroup$ This question is about fluidics and principles of physics rather than that of biology. $\endgroup$ – WYSIWYG Jan 10 at 11:03
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The short answer is yes, the accuracy is affected.

Automatic pipettes are most accurate in the middle of their range. The average error on a pipettes is already about 2%, but this is worse at the top end or bottom end of the pipettes minimal and maximal volume.

More subtly, have you ever tried to pipette 1 uL with a 1 mL pipette? Or even 5 uL. The bore of the pipette is too wide for starters, and the expulsion force/pressure to eject the fluid wouldn’t work very well.

On top of that, the surface tension would seriously hamper any ability to actually manipulate the fluid.

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