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I heard Colchicine doubles the chromosome count; I read about that and I understand why but can you guys explain how?

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Colchicine binds to tublin and therefore inhibits the polymerization of microtuble. That way the build-up of the mitotic spindle, necessary for the separation of the chromosomes in the metaphase of mitosis, is inhibited. Therefore, during cell division, one cell remains without any genetic material, while the other cells receives a double chromosome set. While this leads to cell death for animal cells in both cases, in plant cells multiple chromosome sets can have beneficial effects.

Source

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  • $\begingroup$ Yes I understand that but how do you apply it? $\endgroup$ – Aravind Karthigeyan Jan 8 at 0:42
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    $\begingroup$ Just scroll down in the Wikipedia article: When used to induce polyploidy in plants, colchicine cream is usually applied to a growth point of the plant, such as an apical tip, shoot, or sucker. Also, seeds can be presoaked in a colchicine solution before planting. $\endgroup$ – cako Jan 8 at 2:18
  • $\begingroup$ All in all you might run in several problems with your approach. Do you want to grow bigger berries or just do it for fun. Use of colchicine is no guarantee for "better" plants. The degree of changes cannot be predicted and visual changes may be undetectable. Also colchicine is a toxic chemical you should take care with if you even are able to get some. $\endgroup$ – cako Jan 8 at 2:30
  • $\begingroup$ @AravindKarthigeyan if you are interested in the application of colchicine for agricultural purposes then you may ask a question at Gardening & Landscaping. $\endgroup$ – WYSIWYG Jan 8 at 13:11
  • $\begingroup$ I plan to breed the tetraploid with a regular diploid to get seedless fruits. $\endgroup$ – Aravind Karthigeyan Jan 8 at 23:40

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