I saw 5X bingind buffer maded by;

  • 100mM HEPES, pH 7.6
  • 5mM EDTA
  • 50mM ammonium sulfate
  • 5mM DTT
  • 1% Tween-20
  • 150mM potassium chloride

which reaction helps this buffer? I don't consider to this buffer participates in which experiment by name. Although this buffer have another name but they writes just '5X bonding buffer'..

  • $\begingroup$ From what kind of reaction/protocol did you take this description? $\endgroup$ – Chris Jan 9 at 8:42
  • $\begingroup$ I saw that recipie at pre-made buffer selling site. there's no mentioned about buffer usage. $\endgroup$ – Koreanraichu Jan 9 at 8:45
  • $\begingroup$ Can you give us the link? $\endgroup$ – Chris Jan 9 at 8:55
  • $\begingroup$ biosesang.com/bbs/shop/item.php?it_id=B2003 <<here. Application used by 'Applications: Suitable for molecular biology applications.'. $\endgroup$ – Koreanraichu Jan 10 at 0:53
  • $\begingroup$ The description says "binding buffer" without specifying. This is pretty standard stuff, containing a buffer (Hepes), some salt and detergents. Nothing too special, but I wouldn't use a "one fits all" buffer. $\endgroup$ – Chris Jan 10 at 19:16

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