0
$\begingroup$

So I want to run an experiment with yeast. I want to monitor the population as close as possibly in regards to its size. I am aware I could use a colorimeter or such, but I can only get a microscope. Is there a way I can estimate the population with a microscope?

$\endgroup$
1
$\begingroup$

Determination of cell concentration using a microscope is usually done by using a hemocytometer, also called counting chamber or Neubauer-counting chamber.

Basically, you count the cells in a defined volume within a specific area. This way you can calculate the concentration in your medium and also the total cell count.

I found a good explanation especially for counting yeast cells here.

$\endgroup$
  • $\begingroup$ Is there a way to find the concentration without using a hemocytometer? $\endgroup$ – Brian Blumberg Jan 11 at 5:03
  • $\begingroup$ @BrianBlumberg You can plate the cells after serial dilution and count the colonies. Since you mentioned that you want to use a microscope, you need to use a haemocytometer; you cannot estimate true concentration on a normal slide. Also, haemocytometer is more accurate than OD, if the cells have different sizes. $\endgroup$ – WYSIWYG Jan 11 at 10:24

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.