0
$\begingroup$

So I want to run an experiment with yeast. I want to monitor the population as close as possible in regard to its size. I am aware I could use a colorimeter or such, but I can only get a microscope. Is there a way I can estimate the population with a microscope?

$\endgroup$

1 Answer 1

3
$\begingroup$

Determination of cell concentration using a microscope is usually done by using a hemocytometer, also called counting chamber or Neubauer-counting chamber.

Basically, you count the cells in a defined volume within a specific area. This way you can calculate the concentration in your medium and also the total cell count.

I found a good explanation especially for counting yeast cells here.

$\endgroup$
2
  • $\begingroup$ Is there a way to find the concentration without using a hemocytometer? $\endgroup$ Jan 11, 2019 at 5:03
  • $\begingroup$ @BrianBlumberg You can plate the cells after serial dilution and count the colonies. Since you mentioned that you want to use a microscope, you need to use a haemocytometer; you cannot estimate true concentration on a normal slide. Also, haemocytometer is more accurate than OD, if the cells have different sizes. $\endgroup$
    – WYSIWYG
    Jan 11, 2019 at 10:24

You must log in to answer this question.

Not the answer you're looking for? Browse other questions tagged .