Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if the commonly used Total plate count method with PCA could be improved with the addition of TTC (triphenyl tetrazoliumchloride) for the mesophilic (37C) and thermophilic (55C) test. It began good with the mesophilic test being alright and ready to be validated but with the thermophilic their were allot of weird results. for example I've got a couple of test samples (milkpowder) and incubated it triplo with one half TTC added to it and the other half not. the results were al over the place with not a single monster with the same result. is the method even possible? the reason for this assignment is because during the counting of the samples (total plate count) the powders can be seen for micro-organisms (same color) so if this works it is a improvement of quality. thanks for the answers.

PS: sorry for the bad English and grammatical errors (Dutch student)

  • $\begingroup$ I think you should explain more about what you are trying to do. Mention the protocol in brief. There could be several reasons for why your experiment is not working. It would be difficult for others to troubleshoot if they don't know all the steps. And, what is PCA? $\endgroup$ – WYSIWYG Jan 18 at 17:24

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