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For gel electrophoresis of DNA, we use TAE or TBE. TAE buffer is cheaper than TBE but it has low buffering capacity; it exhausts faster than TBE buffer and there is a need to change the buffer during gel electrophoresis when electrophoresis longer.

but what is 'exhaused' in gel electrophoresis?

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  • $\begingroup$ Do you have a source saying you need to change buffers during electrophoresis? I have never heard this and never seen any problems running gels longer. $\endgroup$ – Chris Jan 24 at 9:02
  • $\begingroup$ Never heard of having to change the buffer during electrophoresis. When you turn down the voltage, you just throw it away. I would even think that replacing the buffer during the test, could produce errors and affect in a negative way the layers separated into the gel. Right now i do not have my labs notes under hand $\endgroup$ – user49173 Jan 24 at 14:12
  • $\begingroup$ OMG I heard about that where BRIC, Korean biologist community. ...I cannot believe there. $\endgroup$ – Koreanraichu Jan 25 at 2:22
  • $\begingroup$ Then, what is 'exhausetd buffer' in gel electrophoresis? $\endgroup$ – Koreanraichu Jan 25 at 2:23

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