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I am hoping that you can help me improve my understanding of transcription. :) I have tried searching for the answers to all of these questions online already, but I have either had trouble finding relevant information or I have had trouble understanding it (i.e. it was too high-level for me). I have also tried to answer my questions by browsing through different maps of genes (like this one: http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr9%3A132847932-133136551&hgsid=709471277_W3A9vdobj2J7obkSXshDnCBnaTeK), but my questions are still unresolved. Please note that I have come up with the following questions in my independent learning - these questions are NOT assigned homework. In all questions, I'm referring to eukaryotic (linear) DNA.
Am I correct to believe that in a single chromosome, one strand of DNA is not the template strand for all genes within that chromosome? For instance, let's call one ssDNA molecule "A" and the complementary ssDNA molecule "B"; together, A and B make up a single chromosome. Am I correct to believe that in a single chromosome, the template strand for some genes will be A, and for other genes will be B?
If my statement in question #1 is correct, then does that mean that multiple genes within a chromosome can be transcribed in opposite directions? Note that when I say direction, I do NOT mean 5' to 3' vs 3' to 5'. What I mean by "direction" is looking from left to right.
Is it true that both strands of the double helix cannot be transcribed for a single gene in question? Is this because the promoter will only be found on one of the strands?
Thank you in advance for your time!