In Sanger sequencing, how can we read the sequence if we have 2 bands with the same size?
closed as off-topic by David, James, kmm, theforestecologist♦, De Novo Feb 22 at 3:16
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It happens if your sample contains a mix of two DNA molecules, with different bases at a certain position.
In your case, you would have a mixture of ACTGGCTA and ACTGTCTA.
In real life, the "double band" would be half as intense as the other single bands, as the signal is "divided by two".