I am working on a project that aims to synthesize a particular molecule from a purely synthetic route, in doing so I am attempting to create a strain of e.coli which would be able to use its trans enoyl isomerase activity (fadB gene) to convert the 2E trans-2-enoyl CoA to its 3E variant. However the 2E is taken up by enoyll CoA hydratase. I thought of inhibiting the enoyl CoA hydratase enzyme but the catch is that it is also created from the fadB gene. Thus gene knock out is not a possibility.
Sincerely, Some french science student