I'm working on a computer visualisation of the inside of a human cell nucleus (embryonic stem cell for now if it makes a difference) and want a good approximation of the chemical composition of the nucleoplasm so I can make a faithful representation (have the correct density and ratio of molecules giggling around in the camera volume).
My assumption is the nucleoplasm would consist of the following in some ratio:
- Ions (Cl-, Na+, K+, Ca+)
- Proteins (6,297 types according to human protein atlas)
- DNA, RNA - as loose strands
- Chromatin (DNA bound to histones) including the 23 chromosome pairs
- Unbound amino acids (22 types)
Would this composition be stable over the life of the cell or fluctuate at different phases?