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Is it possible to use a biologically active Telemorease Elongation Reverse Transcriptase (TERT) in the place of the Reverse Transcriptase (RT) for quantitative Reverse Transcriptase pcr (qRT-pcr)? Yes I do know that it would seemingly have no advantages. What kind of reverse transcriptase is used in qRT-pcr is there a standard like taq dna pol is used for qpcr?

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What kind of reverse transcriptase is used in qRT-pcr is there a standard like taq dna pol is used for qPCR

M-MuLV (Monoley murine leukemia virus) reverse transcriptase

Some RT enzymes are engineered to remove the RNAseH domain so that template RNA persists for more rounds of RT.

Is it possible to use a biologically active Telemorease Elongation Reverse Transcriptase (TERT) in the place of the Reverse Transcriptase

Perhaps it can catalyse an RT reaction in-vitro. But commercial enzymes need to be efficient and I think using TERT won't be that advantageous.

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