High school sudents are going to visit my university and I plan to demonstrate crystallisation of lysozyme. I ordered pure lysozyme from VWR. I can easily crystallise this within 15 min in batch (4% w/v NaCl, 35 mg/mL Lysozyme dissolved in 0.2M NaAc). However, as many different student groups will go through this experiment sequentially, it is preferable that it takes even less time (5 min waiting time maximum). I can of course try to optimise this on my own, but I am wondering if anyone has experience with the absolute fastest way lysozyme forms crystals in a batch setup (to save time and chemicals).
In summary: What are the most optimal buffer/pH/lysozyme concentrations that will give crystals (in a batch experiment) within a very short timeframe (<5-6 min)?