I really need to know what Ercc1-/- / DAT-Cre+ mean. I think the 1st part means that the mice don't have the Ercc1 gene (knockout). But what about DAT-Cre+?
This question arised from reading the following paper: "Inefficient DNA Repair Is an Aging-Related Modifier of Parkinson’s Disease" (Sara Sepe et al).
Ercc1-/- / DAT-Cre+ mice are mentioned several times, For e.g.:
Ercc1−/−DAT-Cre+ mice are viable and normal at birth. At 26 weeks of age, specific deletion of Ercc1 in DA neurons mice elicits reduction in tyrosine hydroxylase (TH) immunoreactivity in the SNpc and in the striatum (Figures 1A and 1B). Aging profoundly exacerbates SNpc DA neurons reduction, as evidenced in 52-week-old Ercc1−/−DAT-Cre+ mice (Figure 1C). Damage is limited in DA neurons and other populations remain unaffected, as indicated by unchanged NeuN immunoreactivity (Figures 1A and 1B). Overall, these findings demonstrate that Ercc1 function and NER are essential to preserve integrity of the DA circuits in time.