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I also just experienced the same thing with my DNA sequences, there are w's, Y', R', s' and k'. This is the very first time I've seen this. I am used to N but not these ones. Please kindly assist with knowing what might have caused these. Thanks in advance.

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These are the so called "DNA ambiguity codes", when the sequencing result allows to bases at the position but it cannot be decided which. This can happen for example when you sequence genomic DNA and you have two different alleles present.

The code is the following (taken from this website):

enter image description here

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  • $\begingroup$ Thank you so much. I was really confused and now you've given me some light. Thanks a million! $\endgroup$ – Audrey Vanya Mar 19 '19 at 16:34
  • $\begingroup$ @AudreyVanya You're welcome. What is easy for one person, is not necessarily for the next. That's why we have this community. $\endgroup$ – Chris Mar 19 '19 at 16:52

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